PCR, polymerase chain reaction, is a conventional method of molecular biology research and experiment, and is also a widely used experimental technology in biology, medicine and clinical fields. It is characterized by the ability to increase the tiny amount of DNA. The PCR laboratory is divided into four areas, and entering each work area must be carried out strictly in a single direction, and different work areas use different work clothes (such as different colors). When leaving each work area, the staff shall not take the work clothes out. The four areas are:

1. Reagent preparation area

2. Sample process area

3. amplification area

4. Product analysis area

the instrument and equipment are required: cooling incubator,freezer,frige, thermostatic chamber,centrifuge,mixer,water bath,laminar flow hood,electrophoresis tank,enzyme instrument,balance,autocalve etc.